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of Alternative Technologies for Freshwater Augumentation in East and
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5.2 Biotechnology-based Wastewater Treatment
Introduction
Ukrainian Academy of Sciences Institute of Colloid and Water Chemistry,
in cooperation with a number of organizations, has developed and
implemented a flow-through biotechnology for wastewater treatment that
uses a sequence of bacteria and hydrobionts (aquatic fauna) to immobilize
contaminant loads. These biotechnologies are widely used in wastewater
treatment, drinking water pretreatment, and purification of rainfall and
surface waters (in lakes, bays, channels, etc.).
Technical Description
While this technology can be specifically adapted for particular groups
of pollutants, this case study will examine the biotechnology proposed for
use in the treatment of wastewater from a yeast production facility. This
technology includes three stages. In the first stage, the effluent is
subjected to anaerobic treatment in three consecutive biological reactors
(concrete submerged tanks, filled with "Vija" fibre produced
from textured nylon with a specially tailored interlacing). The fibres
provide a substrate that approximates sone 10 000 to 100 000 m2 of surface
area/m3 of fibre. The anaerobes that inhabit this surface area can reduce
the COD of the influent wastewater by not less than 80% (as COD). In the
second stage, the effluent is subjected aerobic treatment in a modified
trickling biological filter, wherein the conventional substrate of rocks
has been replaced by additional fibre batting. In this chamber, the
effluent is denitrified to rid the effluent of excess ammonia. In the
third stage, the effluent is subjected to aerobic mineralization using
activated sludge. This stage serves as a final polishing stage in the
enhanced water purification process. When the contaminated water emerges
from this portion of the process, its BOD is reduced from 3 000 mg/l to 6
mg/l.
Extent of Use
Table 6 lists the industrial facilities in which biotechnological
systems of wastewater treatment are in operation.
Operation and Maintenance
Operation and maintenance costs incurred using this technology are 3
times less than the costs of wastewater treatment in aerobic activated
sludge tanks, and half as many staff are required to operate this type of
technology. The types of professional skills, however, are slightly
different to those required in conventional wastewater treatment plants as
one staff member should be a biologist-ecologist (in addition to an
engineering technician and electrician). Because this is a biological
rather than a mechanical system, electric power consumption is reduced by
a factor of 3 times. Likewise, because of the mix of aquatic organisms
used in this treatment process, the amount of activated sludge is reduced
by 5 times. The microorganisms used in the process could be selected from
local sources.
Level of Involvement
This technology is typically implemented by the private sector, when
employed in the factory setting, or by the municipal sector, when used for
community wastewater treatment.
TABLE 6. Application of Biotechnology-based
Wastewater Treatment Systems in Ukraine.

Costs
The capital cost of a biotechnology-based treatment facility with a
capacity of 1 500 m3/day is about $2 430 000 (or $1.62 per unit of
output). Operation and maintenance costs are 3 times less than the cost of
water treatment in aerobic sludge tanks. The fibre batting costs $10/kg.
Purification facilities generally use between 0.3 and 3 kg of this
material/m3 of effluent treated. In most applications, the depreciated
cost of wastewater treatment prior to the introduction of
biotechnological-based treatment was $376.10 (or $0.25 per unit of
output), and, after its implementation, these costs declined to $150.20
(or $0.10 per unit of output). Under ideal conditions, the
biotechnological treatment is self-adjusting once it has been launched.
Effectiveness of the Technology
In urban wastewater treatment applications, these biotechnologies
produce an effluent which improves on the performance of conventional
biological treatment facilities by 50% to 60% overall. Water quality of
the purified water leaving the plant is 5 to 10 times better; typical
output has a COD of between 15 and 20 mg/dm³, a BOD of between 2 and
3 mg/ dm³, and a suspended matter concentration of less than 2 mg/dm³..
Power costs are reduced by 3 to 4 times, and surplus sludge quantities are
reduced by 5 to 8 times.
Advantages
Under ideal conditions, the treatment facility is self-adjusting, and
can be used to purify any wastewater, including almost toxic wastewaters,
to required levels of water purity.
Disadvantages
There are no known disadvantages to the use of biotechnology-based
wastewater treatment methods.
Further Development of the Technology
This biological technology is based upon universal principles inherent
in aquatic ecology. Hence, this type of system can be used for the
treatment of both raw water and wastewater from various industrial and
municipal sources. The basic principles employed are the principles of
spatial succession of different microorganisms and of the nutritional
chain of hydrobionts (the food web). The technology is generally
reagent-free, but, in the case of the technology being used for water
treatment in chemical facilities, reagents might be also used for
pretreatment of the wastewater. Reagents may also be used when there might
be a shortage of biological growth elements in the water being treated.
In industrial waterworks, this reagent-free, inexpensive, and
environmentally-safe treatment method can replace the traditional chemical
(biocide) treatment of recycled water needed to reduce biological growth
within heat-exchangers. In natural waters, this technology can be used to
completely remove up to 100 ml of oil per square meter of water surface
daily.
Information Sources
Dr Vladimir A. Demkin, Ministry of Environmental
Protection and Nuclear Safety, 5 Khreschatyk St., Kyiv-1, Ukraine, Tel.
(380-44) 228 0786, fax: (380-44) 229 8050, e-mail:
demkin@mep.freenet.kiev.ua.
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